Binding of Filamentous Actin and Winding into Fibrillar Aggregates by the Polyphenolic C-Glucosidic Ellagitannin Vescalagin

Authors

  • Prof. Stéphane Quideau,

    Corresponding author
    1. Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
    • Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
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  • Dr. Céline Douat-Casassus,

    1. Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
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  • Daniela Melanie Delannoy López,

    1. Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
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  • Dr. Carmelo Di Primo,

    1. IECB/INSERM U869, 2 rue Robert Escarpit, 33607 Pessac Cedex (France)
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  • Dr. Stefan Chassaing,

    1. Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
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  • Rémi Jacquet,

    1. Université de Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-2215
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  • Dr. Frédéric Saltel,

    1. IECB/INSERM U1053, 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-3076
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    • These authors contributed equally.

  • Dr. Elisabeth Genot

    Corresponding author
    1. IECB/INSERM U1053, 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-3076
    • IECB/INSERM U1053, 2 rue Robert Escarpit, 33607 Pessac Cedex (France), Fax: (+33) 5-4000-3076
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    • These authors contributed equally.


  • Financial support from the Agence Nationale de la Recherche (ANR-06-BLAN-0139 and ANR-06-BLAN-0362), the Conseil Regional d’Aquitaine (grant 20071301020), La Ligue Contre le Cancer (Comité Dordogne 2006 and 2007), and the Institut Européen de Chimie et Biologie (2007 Internal Seed Grant Program) is gratefully acknowledged. D.M.D.L. acknowledges the support of Fundayacucho and the French Embassy in Caracas, Venezuela, for her doctoral research assistantship. We thank A. Nube, A. Labrousse, and I. Maridonneau-Parini for preliminary experiments, R. Boujemaa-Paterski and L. Blanchoin for reagents and precious advice on actin polymerization assays, and A. Grelard for NMR spectroscopic experiments.

Abstract

original image

Winding it up: The plant polyphenolic metabolite vescalagin fulfills all the requirements for use as an antiactin agent in cellular biological investigations. Despite its high hydrophilicity, it rapidly enters cells and disturbs the organization of the actin cytoskeleton in a dose-dependent reversible manner by binding fibrillar actin and forcing the actin filaments (left) to wind themselves into ball-like fibrillar aggregates (right).

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