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Hybrid Ferritin Nanoparticles as Activatable Probes for Tumor Imaging

Authors

  • Dr. Xin Lin,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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    • These authors contributed equally to this work.

  • Dr. Jin Xie,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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    • These authors contributed equally to this work.

  • Lei Zhu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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  • Dr. Seulki Lee,

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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  • Dr. Gang Niu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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  • Dr. Ying Ma,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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  • Dr. Kwangmeyung Kim,

    1. Biomedical Research Center, Korea Institute of Science and Technology, Seoul (Korea)
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  • Dr. Xiaoyuan Chen

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) (USA), Fax: (+1) 301-480-1613
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  • This work was supported by the Intramural Research Program of the National Institute of Biomedical Imaging and Bioengineering, NIH. J.X. is partially supported by the NIH pathway to independence (K99/R00) award; S.L. is partially supported by a NIBIB/NIST fellowship; G.N. is an Imaging Sciences Training Program (ISTP) Fellow. We thank Dr. Henry S. Eden for proofreading the manuscript.

Abstract

original image

Rattling the cage: Protein cages are used as a scaffold to build protease-activatable probes. The self-assembly of ferritin cages generates hybrid proteins with matrix metalloproteinase (MMP)-specific activation (see picture). The formula with the highest activation efficiency is validated as a tumor-specific probe in a xenograft mouse model.

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