We wish to acknowledge the Canadian Institute of Health Research, NSERC, and the Ontario Institute for Cancer Research for their generous support of this work.
Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture†
Article first published online: 6 APR 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 18, pages 4137–4141, April 26, 2011
How to Cite
Vasilyeva, E., Lam, B., Fang, Z., Minden, M. D., Sargent, E. H. and Kelley, S. O. (2011), Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture. Angew. Chem. Int. Ed., 50: 4137–4141. doi: 10.1002/anie.201006793
- Issue published online: 20 APR 2011
- Article first published online: 6 APR 2011
- Manuscript Revised: 2 JAN 2011
- Manuscript Received: 28 OCT 2010
- Canadian Institute of Health Research
- Ontario Institute for Cancer Research
- nucleic acids
A chip-based platform is reported that is able to detect as few as 10 cancer cells. By developing sub-milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer-specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.