Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture


  • We wish to acknowledge the Canadian Institute of Health Research, NSERC, and the Ontario Institute for Cancer Research for their generous support of this work.


original image

A chip-based platform is reported that is able to detect as few as 10 cancer cells. By developing sub-milliscale sensors that are able to capture slow moving biological targets with high efficiency (see picture; scale bar 50 μm), cancer-specific sequences were detected in crude lysates of leukemia cells. This achievement relied on the development of a new type of molecular probe that improves the solubility and performance of neutral nucleic acids.