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Label-Free Detection of Few Copies of DNA with Carbon Nanotube Impedance Biosensors

Authors

  • Tetiana Kurkina,

    1. Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
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  • Dr. Alexis Vlandas,

    1. Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
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  • Dr. Ashraf Ahmad,

    1. Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
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  • Prof. Klaus Kern,

    1. Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
    2. Institut de Physique de la Matière Condensée, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
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  • Dr. Kannan Balasubramanian

    Corresponding author
    1. Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
    • Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, 70569 Stuttgart (Germany), Fax: (+49) 711-689-1662
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  • This project was funded by the German Federal Ministry of Education and Research (BMBF, ID: O3X5516). We thank S. Schmid (Technology Group) for help with photoresists; V. Pachauri for SEM imaging; and Dr. K. Heimann (Karlsruhe Institute of Technology) and Prof. C. Richert (University of Stuttgart) for providing us with gold-nanoparticle-decorated DNA.

Abstract

original image

Finding that needle in the haystack: A label-free on-chip detection strategy based on carbon nanotubes (see picture) was used to detect an oligonucleotide target sequence of which less than 2000 copies were present in a 30 μL sample droplet, without the need for PCR. Instead, sensitive low-noise impedance measurement coupled to field-effect detection enabled attomolar DNA detection in a heterogeneous environment.

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