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Keywords:

  • NMR spectroscopy;
  • paramagnetic relaxation enhancement;
  • proteins;
  • structural biology
Thumbnail image of graphical abstract

The solvent helps out: Conventional protocols for structural analysis of protein complexes often fail if only sparse experimental data are available. NMR-spectroscopy-derived solvent paramagnetic relaxation enhancements (PREs) from a soluble spin label (picture) significantly improve the structural quality of a 150 kDa protein complex. This general protocol is an efficient approach for structural analysis of large protein complexes in solution.