Dynamic and Programmable DNA-Templated Boronic Ester Formation

Authors

  • Anthony R. Martin,

    1. Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
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  • Dr. Ivan Barvik,

    1. Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2 (Czech Republic)
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  • Dr. Delphine Luvino,

    1. Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
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  • Dr. Michael Smietana,

    Corresponding author
    1. Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
    • Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
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  • Dr. Jean-Jacques Vasseur

    Corresponding author
    1. Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
    • Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS–Universités Montpellier 1 et 2, Place Bataillon, 34095 Montpellier (France), Fax: (+33) 4-6714-2029
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  • The MENRT (A.R.M.), the Région Languedoc-Roussillon (D.L.), and the CNRS are gratefully acknowledged for financial support. I.B. thanks the Ministry of Education, Youth and Sports of the Czech Republic (project no. MSM 0021620835). We thank Dr. C. Mari for helpful discussions.

Abstract

original image

Plug and play: A helix with a boronate ester linkage was constructed by DNA- or RNA-templated assembly through the plugging together of appropriately designed half-sequences, one with a boronic acid at its 5′ end, the other with a ribonucleotide at its 3′ end. The two hybridization steps in the three-component assembly (see picture) could be controlled reversibly by external stimuli, including an acid or base, cyanide ions, or fructose.

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