These authors contributed equally.
Superresolution Imaging of Albumin-Conjugated Fluorescent Nanodiamonds in Cells by Stimulated Emission Depletion†
Article first published online: 2 FEB 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 10, pages 2262–2265, March 1, 2011
How to Cite
Tzeng, Y.-K., Faklaris, O., Chang, B.-M., Kuo, Y., Hsu, J.-H. and Chang, H.-C. (2011), Superresolution Imaging of Albumin-Conjugated Fluorescent Nanodiamonds in Cells by Stimulated Emission Depletion. Angew. Chem. Int. Ed., 50: 2262–2265. doi: 10.1002/anie.201007215
This research was supported by the Academia Sinica and the National Science Council, Taiwan with Grant No. 99-2119M-001-026-. We thank Dr. K. Y. Han and Dr. C. Eggeling at the Max Planck Institute for Biophysical Chemistry in Göttingen (Germany) for instruction and assistance in setting up the STED system.
- Issue published online: 24 FEB 2011
- Article first published online: 2 FEB 2011
- Manuscript Received: 17 NOV 2010
- Academia Sinica
- National Science Council, Taiwan. Grant Number: 99-2119M-001-026-
- STED microscopy
A bunch of loners: Fluorescent nanodiamonds (FNDs) noncovalently conjugated with bovine serum albumin (BSA) or α-lactalbumin exhibited good dispersibility in a buffer with only minor or no agglomeration. They are useful as photostable fluorescent markers in cells for superresolution imaging by STED (see confocal fluorescence image of an FND-labeled cell (left) and STED image of single BSA-conjugated FNDs (right)).