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Superresolution Imaging of Albumin-Conjugated Fluorescent Nanodiamonds in Cells by Stimulated Emission Depletion

Authors

  • Yan-Kai Tzeng,

    1. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106 (Taiwan), Fax: (+886) 2-2362-0200
    2. Department of Chemistry, National Taiwan University, Taipei, 106 (Taiwan)
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    • These authors contributed equally.

  • Dr. Orestis Faklaris,

    1. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106 (Taiwan), Fax: (+886) 2-2362-0200
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    • These authors contributed equally.

  • Be-Ming Chang,

    1. Department of Chemistry, National Taiwan Normal University, Taipei, 106 (Taiwan)
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  • Yung Kuo,

    1. Department of Chemistry, National Taiwan University, Taipei, 106 (Taiwan)
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  • Dr. Jui-Hung Hsu,

    1. Department of Materials Science and Optoelectronic Science, National Sun Yat-Sen University, Kaohsiung 804 (Taiwan)
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  • Dr. Huan-Cheng Chang

    Corresponding author
    1. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106 (Taiwan), Fax: (+886) 2-2362-0200
    2. Department of Chemistry, National Taiwan University, Taipei, 106 (Taiwan)
    3. Department of Chemistry, National Taiwan Normal University, Taipei, 106 (Taiwan)
    • Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106 (Taiwan), Fax: (+886) 2-2362-0200
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  • This research was supported by the Academia Sinica and the National Science Council, Taiwan with Grant No. 99-2119M-001-026-. We thank Dr. K. Y. Han and Dr. C. Eggeling at the Max Planck Institute for Biophysical Chemistry in Göttingen (Germany) for instruction and assistance in setting up the STED system.

Abstract

original image

A bunch of loners: Fluorescent nanodiamonds (FNDs) noncovalently conjugated with bovine serum albumin (BSA) or α-lactalbumin exhibited good dispersibility in a buffer with only minor or no agglomeration. They are useful as photostable fluorescent markers in cells for superresolution imaging by STED (see confocal fluorescence image of an FND-labeled cell (left) and STED image of single BSA-conjugated FNDs (right)).

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