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An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification

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  • We thank the NSF of China (Nos. 20725621, 20876024, 21076032, 21072024, and 21006009), the National Basic Research Program of China (2009CB724706), and the Cultivation Fund of the Key Scientific and Technical Innovation Project (707016) for support of this work. We also thank Dr. Richard W. Horobin for advice on the estimation of logP values.

Abstract

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Seeing red: DEAB-TO-3 shows a large fluorescence enhancement upon binding to native DNA and a distinct selectivity for double-stranded DNA over total RNA. As a red fluorescent live-cell-permeant DNA minor groove binder, DEAB-TO-3 is promising for highly sensitive DNA detection in vitro and nucleus-specific imaging and DNA quantification in vivo (see picture).

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