Financial support from the European Research Council under the Seventh Framework Programme (FP7/2007–2013)/ERC of the European Community (Starting Grant agreement no. 239898) is acknowledged.
Detection of Enzyme Activity through Catalytic Signal Amplification with Functionalized Gold Nanoparticles†
Article first published online: 31 JAN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 10, pages 2307–2312, March 1, 2011
How to Cite
Bonomi, R., Cazzolaro, A., Sansone, A., Scrimin, P. and Prins, L. J. (2011), Detection of Enzyme Activity through Catalytic Signal Amplification with Functionalized Gold Nanoparticles. Angew. Chem. Int. Ed., 50: 2307–2312. doi: 10.1002/anie.201007389
- Issue published online: 24 FEB 2011
- Article first published online: 31 JAN 2011
- Manuscript Received: 24 NOV 2010
- European Research Council. Grant Number: 239898
- signal amplification
A cascade of two catalytic events was used to detect enzyme activity: When a peptide substrate acting as an inhibitor for a catalytic gold nanoparticle was hydrolyzed by an enzyme, the catalytic activity of the nanoparticle was restored, and a large amount of a yellow reporter molecule was produced (see picture; S=substrate, P=product). The assay can be made selective for a particular enzyme by changing the inhibitory peptide.