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DNA as a Molecular Ruler: Interrogation of a Tandem SH2 Domain with Self-Assembled, Bivalent DNA–Peptide Complexes

Authors

  • Hendrik Eberhard,

    1. Department of Chemistry, Humboldt University Berlin, Brook-Taylor-Strasse 2, 12489 Berlin (Germany), Fax: (+49) 30-2093-7266
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  • Franziska Diezmann,

    1. Department of Chemistry, Humboldt University Berlin, Brook-Taylor-Strasse 2, 12489 Berlin (Germany), Fax: (+49) 30-2093-7266
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  • Prof. Dr. Oliver Seitz

    Corresponding author
    1. Department of Chemistry, Humboldt University Berlin, Brook-Taylor-Strasse 2, 12489 Berlin (Germany), Fax: (+49) 30-2093-7266
    • Department of Chemistry, Humboldt University Berlin, Brook-Taylor-Strasse 2, 12489 Berlin (Germany), Fax: (+49) 30-2093-7266
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  • This research was supported by the Deutsche Forschungsgemeinschaft (SFB 765). We thank Prof. Dr. Gabriel Waksman, University College London, for providing plasmids for GST-Syk-tSH2 expression.

Abstract

original image

Two peptides on display: The self-assembly of DNA complexes enables the bivalent presentation of phosphopeptides. Flexibility and distance in the ligand arrangement can be adjusted through the choice of appropriate DNA templates. Spatial screening of the tandem SH2 domain of Syk kinase with these probes (see picture) indicated the accessible arrangements of the two homologous binding pockets and the flexibility of the connecting protein linker.

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