Photoactivatable and Cell-Membrane-Permeable Phosphatidylinositol 3,4,5-Trisphosphate

Authors

  • Dr. Matthias Mentel,

    1. Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany), Fax: (+49) 6221-387-206
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  • Dr. Vibor Laketa,

    1. Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany), Fax: (+49) 6221-387-206
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  • Dr. Devaraj Subramanian,

    1. Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany), Fax: (+49) 6221-387-206
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  • Dr. Hartmut Gillandt,

    1. Sirius Fine Chemicals (SiChem) GmbH, Fahrenheitstrasse 1, 28359 Bremen (Germany)
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  • Dr. Carsten Schultz

    Corresponding author
    1. Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany), Fax: (+49) 6221-387-206
    • Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg (Germany), Fax: (+49) 6221-387-206
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  • We thank the Advanced Light Microscopy Facility at EMBL and Heike Stichnoth for providing cells. Funding was partially provided by SBCancer, the ESF, and the DFG(Schu843/8-1). M.M. is a fellow of the EMBL interdisciplinary postdoc program (EIPOD).

Abstract

original image

Unleashed inside cells: The signaling molecule PI(3,4,5)P3 is prepared in a form that allows its cellular uptake and subsequent activation by light (see picture). The rapid eight-step synthetic sequence yields a stable derivative of the signaling molecule with high biological activity after deprotection (“uncaging”) with a laser flash, in live cells.

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