Quantitative 3D Analysis of Nitric Oxide Diffusion in a 3D Artery Model Using Sensor Particles


  • We thank Dr. M. Kino-oka and Dr. M.-H. Kim at Osaka University for CLSM observation. This work was supported mainly by PRESTO-JST, partly by an Industrial Technology Research Grant Program in 2006 (06B44017a) from NEDO of Japan, a Grant-in-Aid for Scientific Research on Innovative Areas (21106514) from MEXT of Japan, and by the Noguchi Institute.


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NO blood lost: Three-dimensional analysis of nitric oxide (NO) diffusion in a blood vessel in response to drug stimulation was achieved by using 3D artery models including NO sensor particles (see picture). The quantity and diffusion distance of NO in the five-layered models were almost the same as those for the in vivo blood vessel response. This method enables an in vitro bioassay of tissue responses instead of animal experiments.