M.M.S. thanks the EPSRC and ERC grant “Naturale” for funding. We gratefully acknowledge stimulating discussions with Bruce Cohen at the Molecular Foundry (LBNL). FRET=Förster resonance energy transfer.
Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity†
Article first published online: 9 MAR 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 15, pages 3417–3420, April 4, 2011
How to Cite
Ghadiali, J. E., Lowe, S. B. and Stevens, M. M. (2011), Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity. Angew. Chem. Int. Ed., 50: 3417–3420. doi: 10.1002/anie.201008263
- Issue published online: 31 MAR 2011
- Article first published online: 9 MAR 2011
- Manuscript Received: 30 DEC 2010
Straightforward and label-free: A simple method for the detection of histone-modifying enzymes is based on quantum dot (QD) Förster resonance energy transfer (FRET) donors. Histone acetyltransferase activity can be measured via the enzyme-dependent assembly of a quantum dot/peptide immunocomplex (see picture; His=histidine), which can be conducted in a homogeneous reaction without the need for complicated surface derivatization procedures.