A Branched Extender Unit Shared between Two Orthogonal Polyketide Pathways in an Endophyte

Authors

  • Dr. Zhongli Xu,

    1. Leibniz Institute for Natural Product Research and Infection Biology, HKI, Department of Biomolecular Chemistry, Beutenbergstrasse 11a, 07745 Jena (Germany), Fax: (+49) 3641-532-0804
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    • These authors contributed equally to this study.

  • Dr. Ling Ding,

    1. Leibniz Institute for Natural Product Research and Infection Biology, HKI, Department of Biomolecular Chemistry, Beutenbergstrasse 11a, 07745 Jena (Germany), Fax: (+49) 3641-532-0804
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    • These authors contributed equally to this study.

  • Prof. Dr. Christian Hertweck

    Corresponding author
    1. Leibniz Institute for Natural Product Research and Infection Biology, HKI, Department of Biomolecular Chemistry, Beutenbergstrasse 11a, 07745 Jena (Germany), Fax: (+49) 3641-532-0804
    2. Friedrich Schiller University, Jena (Germany)
    • Leibniz Institute for Natural Product Research and Infection Biology, HKI, Department of Biomolecular Chemistry, Beutenbergstrasse 11a, 07745 Jena (Germany), Fax: (+49) 3641-532-0804
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  • We thank A. Perner and F. Rhein for MS and NMR spectroscopy measurements, B. Kuehn, H. Heinecke, and U. Valentin for assistance in routine experiments, and C. Weigel for performing biological assays. This research was financially supported by the BMBF (GenoMik Transfer).

Abstract

original image

Sharing a branch: The first biosynthesis of an unprecedented branched PKS extender unit from valine/isobutyrate was unveiled, involving a designated ketosynthase III and a crotonyl reductase/carboxylase (CCR). Isobutylmalonyl-CoA (ibMCoA) is not only employed in divergolide biosynthesis but is also incorporated into novel, antibacterial germicidins (see scheme). Such natural biocombinatorics are unparalleled in polyketide biosynthesis.

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