SEARCH

SEARCH BY CITATION

Keywords:

  • click chemistry;
  • enzyme catalysis;
  • glycoconjugates;
  • glycosylation;
  • transferases
Thumbnail image of graphical abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).