Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo

Authors

  • Tianqing Zheng,

    1. Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
    Search for more papers by this author
  • Dr. Hao Jiang,

    1. Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
    Search for more papers by this author
  • Dr. Marilyn Gros,

    1. Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
    Search for more papers by this author
  • Dr. David Soriano del Amo,

    1. Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
    Search for more papers by this author
  • Subha Sundaram,

    1. Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
    Search for more papers by this author
  • Prof. Grégoire Lauvau,

    1. Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
    Search for more papers by this author
  • Prof. Florence Marlow,

    1. Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
    Search for more papers by this author
  • Dr. Yi Liu,

    1. The Molecular Foundry, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley (USA)
    Search for more papers by this author
  • Prof. Pamela Stanley,

    1. Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
    Search for more papers by this author
  • Prof. Peng Wu

    Corresponding author
    1. Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
    • Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
    Search for more papers by this author

  • This work was supported by the National Institutes of Health (R01GM093282 to P.W. and R01CA036434 to P.S.) and the Mizutani Foundation for Glycoscience (to P.W.). Y.L. was supported by the Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under contract No. DE-AC02-05 CH11231. We thank Prof. Carolyn Bertozzi for providing DIFO-647. Two disaccharides used in the kinetic assay were provided by The Consortium for Functional Glycomics (funded by NIGMS-GM62116).

Abstract

original image

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Ancillary