• Open Access

Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)

Authors

  • Dr. F. Persson,

    1. Department of Physics, University of Gothenburg, Fysikgränd 3, 412 96 Gothenburg (Sweden)
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    • These authors contributed equally.

  • P. Bingen,

    1. Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210
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    • These authors contributed equally.

  • Dr. T. Staudt,

    1. Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210
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  • Dr. J. Engelhardt,

    1. Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210
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  • Prof. J. O. Tegenfeldt,

    1. Department of Physics, University of Gothenburg, Fysikgränd 3, 412 96 Gothenburg (Sweden)
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  • Prof. Stefan W. Hell

    Corresponding author
    1. Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210
    • Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210
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  • This work was supported by the EU’s 7th Framework Programme (7RP/2007-2013) under grant agreement No. 201418 (READNA), the Swedish Research Council grants 2007-584 and 2007-4454, a Young Investigators’ Grant from HSFP (RGY0078/2007-C) and a AFR PhD grant from the National Research Fund, Luxembourg (TR-PHD BFR08-059) and we thank Christoph Muus for preparing DNA samples.

Abstract

original image

Clearing the haze: Stimulated emission depletion (STED) nanoscopy on single DNA strands reveals details with an up to five- to sixfold improved resolution over confocal microscopy (see images). Thus STED nanoscopy allows features to be distinguished down to the persistence length of DNA (ca. 50 nm) without promoting any significant additional photodamage, such as photobleaching or photonicking.

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