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Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)†

  1. Dr. F. Persson1,‡,
  2. P. Bingen2,‡,
  3. Dr. T. Staudt2,
  4. Dr. J. Engelhardt2,
  5. Prof. J. O. Tegenfeldt1,
  6. Prof. Stefan W. Hell2,*

Article first published online: 6 MAY 2011

DOI: 10.1002/anie.201100371

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 24, pages 5581–5583, June 6, 2011

Additional Information

How to Cite

Persson, F., Bingen, P., Staudt, T., Engelhardt, J., Tegenfeldt, J. O. and Hell, S. W. (2011), Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED). Angewandte Chemie International Edition, 50: 5581–5583. doi: 10.1002/anie.201100371

Author Information

  1. 1

    Department of Physics, University of Gothenburg, Fysikgränd 3, 412 96 Gothenburg (Sweden)

  2. 2

    Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210

  1. ‡

    These authors contributed equally.

*Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg (Germany), Fax: (+49) 6221-54-51210

  1. †

    This work was supported by the EU’s 7th Framework Programme (7RP/2007-2013) under grant agreement No. 201418 (READNA), the Swedish Research Council grants 2007-584 and 2007-4454, a Young Investigators’ Grant from HSFP (RGY0078/2007-C) and a AFR PhD grant from the National Research Fund, Luxembourg (TR-PHD BFR08-059) and we thank Christoph Muus for preparing DNA samples.

Publication History

  1. Issue published online: 1 JUN 2011
  2. Article first published online: 6 MAY 2011
  3. Manuscript Received: 15 JAN 2011

Funded by

  • Funded Access
  • EU’s 7th Framework Programme. Grant Number: 7RP/2007-2013) under grant agreement No. 201418 (READNA)
  • Swedish Research Council
  • HSFP. Grant Number: RGY0078/2007-C
  • National Research Fund, Luxembourg. Grant Number: TR-PHD BFR08-059

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Keywords:

  • DNA;
  • dyes/pigments;
  • fluorescence;
  • single-molecule studies;
  • stimulated emission depletion (STED)

Graphical Abstract

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Clearing the haze: Stimulated emission depletion (STED) nanoscopy on single DNA strands reveals details with an up to five- to sixfold improved resolution over confocal microscopy (see images). Thus STED nanoscopy allows features to be distinguished down to the persistence length of DNA (ca. 50 nm) without promoting any significant additional photodamage, such as photobleaching or photonicking.

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