This work was supported by the Natural Sciences and Research Council of Canada (NSERC) and Sentinel Bioactive Paper Network. Y.L. is a Canada Research Chair.
Fluorogenic DNAzyme Probes as Bacterial Indicators†
Article first published online: 15 MAR 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 16, pages 3751–3754, April 11, 2011
How to Cite
Ali, M. M., Aguirre, S. D., Lazim, H. and Li, Y. (2011), Fluorogenic DNAzyme Probes as Bacterial Indicators. Angew. Chem. Int. Ed., 50: 3751–3754. doi: 10.1002/anie.201100477
- Issue published online: 5 APR 2011
- Article first published online: 15 MAR 2011
- Manuscript Received: 19 JAN 2011
- Natural Sciences and Research Council of Canada (NSERC)
- Sentinel Bioactive Paper Network
- bacterial detection;
- DNA cleavage;
Lighting up bacteria: An RNA-cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluorophore (F) and a quencher (Q).