Communication

Chemical Evolution of a Bacterium’s Genome†

  1. Dr. Philippe Marlière1,
  2. Julien Patrouix2,
  3. Dr. Volker Döring2,
  4. Prof. Dr. Piet Herdewijn3,
  5. Sabine Tricot2,4,5,
  6. Dr. Stéphane Cruveiller2,4,5,
  7. Dr. Madeleine Bouzon2,
  8. Prof. Dr. Rupert Mutzel6,*

Article first published online: 27 JUN 2011

DOI: 10.1002/anie.201100535

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 31, pages 7109–7114, July 25, 2011

Additional Information

How to Cite

Marlière, P., Patrouix, J., Döring, V., Herdewijn, P., Tricot, S., Cruveiller, S., Bouzon, M. and Mutzel, R. (2011), Chemical Evolution of a Bacterium’s Genome. Angew. Chem. Int. Ed., 50: 7109–7114. doi: 10.1002/anie.201100535

Author Information

  1. 1

    Heurisko USA Inc., Delaware (USA)

  2. 2

    CEA, DSV, IG, Genoscope, Evry (France)

  3. 3

    Katholieke Universiteit Leuven, Rega Institute for Medical Research, Laboratory of Medicinal Chemistry, Leuven (Belgium)

  4. 4

    CNRS, UMR Génomique Métabolique

  5. 5

    Université Evry Val d'Essonne (France)

  6. 6

    Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Königin-Luise-Strasse 12–16, 14195 Berlin (Germany), Fax: (+30) 8385-7773

*Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Königin-Luise-Strasse 12–16, 14195 Berlin (Germany), Fax: (+30) 8385-7773

  1. †

    We are grateful to Jean Weissenbach for his constant support and encouragement. We also thank Sophie Tuffet for DNA-composition analysis; Julie Poulain, Karine Labadie, Valérie Barbe, and Béatrice Chane-Woon-Ming for genome sequencing and annotation; Valérie Delmas for strain construction; Isabelle Boko and Angela Lahrz for technical assistance; and Susan Cure, Sven Panke, and Phil Holliger for improving the manuscript.

Publication History

  1. Issue published online: 21 JUL 2011
  2. Article first published online: 27 JUN 2011
  3. Manuscript Revised: 15 APR 2011
  4. Manuscript Received: 21 JAN 2011

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Keywords:

  • chemical evolution;
  • chlorouracil;
  • DNA;
  • nucleic acids;
  • xenobiology
Thumbnail image of graphical abstract

Automated selection was used to evolve an Escherichia coli strain unable to synthesize thymine nucleotides into a chemically modified organism whose DNA genome is composed of adenine, cytosine, guanine, and an artificial base, the thymine analogue 5-chlorouracil. Evolving cells were initially observed as irregular filaments and progressively recovered the appearance of short rods typical of wild-type E. coli (see picture).

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