We are grateful to Jean Weissenbach for his constant support and encouragement. We also thank Sophie Tuffet for DNA-composition analysis; Julie Poulain, Karine Labadie, Valérie Barbe, and Béatrice Chane-Woon-Ming for genome sequencing and annotation; Valérie Delmas for strain construction; Isabelle Boko and Angela Lahrz for technical assistance; and Susan Cure, Sven Panke, and Phil Holliger for improving the manuscript.
Chemical Evolution of a Bacterium’s Genome†
Article first published online: 27 JUN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 31, pages 7109–7114, July 25, 2011
How to Cite
Marlière, P., Patrouix, J., Döring, V., Herdewijn, P., Tricot, S., Cruveiller, S., Bouzon, M. and Mutzel, R. (2011), Chemical Evolution of a Bacterium’s Genome. Angew. Chem. Int. Ed., 50: 7109–7114. doi: 10.1002/anie.201100535
- Issue published online: 21 JUL 2011
- Article first published online: 27 JUN 2011
- Manuscript Revised: 15 APR 2011
- Manuscript Received: 21 JAN 2011
- chemical evolution;
- nucleic acids;
Automated selection was used to evolve an Escherichia coli strain unable to synthesize thymine nucleotides into a chemically modified organism whose DNA genome is composed of adenine, cytosine, guanine, and an artificial base, the thymine analogue 5-chlorouracil. Evolving cells were initially observed as irregular filaments and progressively recovered the appearance of short rods typical of wild-type E. coli (see picture).