Tracing the Hydrogen Source of Hydrocarbons Formed by Vanadium Nitrogenase

Authors

  • Chi Chung Lee,

    1. Molecular Biology & Biochemistry, University of California, Irvine, 2236/2448 McGaugh Hall, Irvine, CA 92697-3900 (USA), Fax: (+1) 949-824-8551
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  • Dr. Yilin Hu,

    Corresponding author
    1. Molecular Biology & Biochemistry, University of California, Irvine, 2236/2448 McGaugh Hall, Irvine, CA 92697-3900 (USA), Fax: (+1) 949-824-8551
    • Molecular Biology & Biochemistry, University of California, Irvine, 2236/2448 McGaugh Hall, Irvine, CA 92697-3900 (USA), Fax: (+1) 949-824-8551
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  • Prof. Dr. Markus W. Ribbe

    Corresponding author
    1. Molecular Biology & Biochemistry, University of California, Irvine, 2236/2448 McGaugh Hall, Irvine, CA 92697-3900 (USA), Fax: (+1) 949-824-8551
    • Molecular Biology & Biochemistry, University of California, Irvine, 2236/2448 McGaugh Hall, Irvine, CA 92697-3900 (USA), Fax: (+1) 949-824-8551
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  • We thank Prof. Dr. D. C. Rees and Dr. N. Dalleska of Caltech (Pasadena) for help on the GC–MS analysis. This work was supported by the Herman Frasch Foundation (grant number 617-HF07; M.W.R.).

Abstract

original image

Hydrocarbons from CO: The vanadium-nitrogenase-catalyzed reduction of carbon monoxide involves the adenosine triphosphate (ATP)-dependent protonation of CO and the subsequent formation of C[BOND]C bonds, leading to the production of small hydrocarbons, such as C2H4, C2H6, C3H6, and C3H8 (see picture). Isotope-substitution studies monitored by GC–MS analysis show that protons are the source of hydrogen for the CO reduction.

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