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Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

Authors

  • Ivan Krstić,

    1. Institute of Physical and Theoretical Chemistry and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main (Germany), Fax: (+49) 69-798-29-404 http://www.prisner.de
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    • These authors contributed equally to this work.

  • Robert Hänsel,

    1. Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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    • These authors contributed equally to this work.

  • Dr. Olga Romainczyk,

    1. Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main (Germany)
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  • Dr. Joachim W. Engels,

    1. Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main (Germany)
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  • Dr. Volker Dötsch,

    1. Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Dr. Thomas F. Prisner

    Corresponding author
    1. Institute of Physical and Theoretical Chemistry and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main (Germany), Fax: (+49) 69-798-29-404 http://www.prisner.de
    • Institute of Physical and Theoretical Chemistry and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt am Main (Germany), Fax: (+49) 69-798-29-404 http://www.prisner.de
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  • Financial support from the Cluster of Excellence Frankfurt “Macromolecular Complexes” is gratefully acknowledged. R.H. thanks the FCI (Fonds der chemischen Industrie) for funding.

Abstract

original image

Mapping the structure of nucleic acids: Pulsed electron–electron double-resonance (PELDOR) spectroscopy has been applied for the first time to map the global structure of nucleic acids inside Xenopus laevis oocytes (see picture). The distances measured in vitro and in cells are the same, which implies the existence of stable overall conformations of the hairpin RNA and the neomycin-sensing riboswitch studied.

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