Protein Chemical Synthesis by Ligation of Peptide Hydrazides


  • This work was supported by NSFC (no. 20802040 and no. 20932006) and the national “973” grants from the Ministry of Science and Technology (no. 2011CB965300).


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pH determines selectivity: The ligation of peptide hydrazides is a new method for protein chemical synthesis that is complementary to native chemical ligation. Peptide hydrazides may be the long-sought reagent equivalent to a “thioester synthon”, one that is stable to the conditions of native chemical ligation.