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Coupling Amperometry and Total Internal Reflection Fluorescence Microscopy at ITO Surfaces for Monitoring Exocytosis of Single Vesicles

Authors

  • Anne Meunier,

    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
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  • Ouardane Jouannot,

    1. CNRS/Université Paris Descartes UMR8192, 45 rue des Saints-Pères, 75270 Paris cedex 06 (France)
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  • Dr. Rémy Fulcrand,

    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
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  • Dr. Isabelle Fanget,

    1. CNRS/Université Paris Descartes UMR8192, 45 rue des Saints-Pères, 75270 Paris cedex 06 (France)
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  • Dr. Marine Bretou,

    1. CNRS/Université Paris Descartes UMR8192, 45 rue des Saints-Pères, 75270 Paris cedex 06 (France)
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  • Dr. Erdem Karatekin,

    1. CNRS/Université Paris Descartes UMR8192, 45 rue des Saints-Pères, 75270 Paris cedex 06 (France)
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  • Dr. Stéphane Arbault,

    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
    2. Present address: Groupe Nanosystèmes Analytiques, Institut des Sciences Moléculaires UMR 5255, Université de Bordeaux (France)
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  • Dr. Manon Guille,

    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
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  • Dr. François Darchen,

    1. CNRS/Université Paris Descartes UMR8192, 45 rue des Saints-Pères, 75270 Paris cedex 06 (France)
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  • Dr. Frédéric Lemaître,

    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
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  • Prof. Christian Amatore

    Corresponding author
    1. Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
    • Département de Chimie, Ecole Normale Supérieure UMR 8640 (CNRS-ENS-UPMC Univ Paris 06), 24 rue Lhomond, 75005 Paris (France), Fax: (+33) 1-4432-3863
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  • This work was supported in part by the CNRS (UMR 8640, FR2702, LIAXiamENS), Ecole Normale Supérieure, French Ministry of Research, ANR (μPHYSCHEMBIO), Université Pierre & Marie Curie Paris 06, and by the European Commission under the Seventh Framework Programme (Nanoscale CP-FP 214566-2). ITO=indium tin oxide.

Abstract

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More transparency in bioanalysis: A microdevice based on transparent indium tin oxide (ITO) electrodes allows simultaneous total internal reflection fluorescence microscopy and amperometric measurements. Use of the device in the coupled optical and electrochemical detection of single exocytotic events is demonstrated with enterochromaffin BON cells (see picture).

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