We gratefully acknowledge financial support from the Beckman Foundation (Beckman Young Investigator Award to A.D.), Research Corporation (Cottrell Scholar Award to A.D.), and the NIH (GM079114). We thank P. G. Schultz (TSRI) for the pEVOL plasmid and D. J. Segal (UC Davis) for the pSSA plasmid.
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Communication
Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease†
Article first published online: 10 JUN 2011
DOI: 10.1002/anie.201101157
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Chou, C. and Deiters, A. (2011), Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease. Angew. Chem. Int. Ed., 50: 6839–6842. doi: 10.1002/anie.201101157
- †
Publication History
- Issue published online: 13 JUL 2011
- Article first published online: 10 JUN 2011
- Manuscript Received: 15 FEB 2011
Funded by
- Beckman Foundation
- Research Corporation
- NIH. Grant Number: GM079114
Keywords:
- caged compounds;
- gene technology;
- light-activation;
- mutagenesis;
- zinc-finger nucleases
Light, zinc-finger nuclease, action: Using unnatural amino acid mutagenesis, a light-activated zinc-finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc-finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).