Highly Selective Fluorescent Probe for Vicinal-Dithiol-Containing Proteins and In Situ Imaging in Living Cells

Authors

  • Chusen Huang,

    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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    • These authors contributed equally.

  • Qin Yin,

    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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    • These authors contributed equally.

  • Prof. Dr. Weiping Zhu,

    Corresponding author
    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
    • State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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  • Prof. Dr. Yi Yang,

    Corresponding author
    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
    • State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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  • Xin Wang,

    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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  • Prof. Dr. Xuhong Qian,

    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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  • Prof. Dr. Yufang Xu

    Corresponding author
    1. State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
    • State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237 (China)
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  • We are grateful for financial support from the National Natural Science Foundation of China (grants 90713026, 21076077), the National Basic Research Program of China (973 Program, 2010CB126100), the National High Technology Research and Development Program of China (863 Program, 2011AA10A207), the China 111 Project (grant B07023), the Fok Ying Tung Education Foundation (grant No. 111022), the Shanghai Leading Academic Discipline Project (B507), and the Fundamental Research Funds for the Central Universities. We also thank Prof. Dr. Charles Yang for the improvement of this article.

Abstract

original image

It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

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