Modular Assembly of Macrocyclic Organo–Peptide Hybrids Using Synthetic and Genetically Encoded Precursors


  • This work was supported by startup funds from the University of Rochester and a NSF Graduate Fellowship to J.M.S. The authors thank Prof. Peter Schultz for kindly providing the pEVOL vector encoding for the mutant MjtRNA and MjTyrRS.


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Mix, click, cyclize: Conformationally constrained organo–peptide hybrids can be constructed by a tandem chemoselective reaction between a synthetic molecule and a recombinant protein. Diverse macrocyclic structures were obtained in cyclic, lariat, and protein-tethered configurations by varying the nature of the synthetic and biosynthetic precursors.