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Graphene Oxide–Peptide Conjugate as an Intracellular Protease Sensor for Caspase-3 Activation Imaging in Live Cells

Authors

  • Dr. Haibo Wang,

    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • Dr. Qing Zhang,

    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • Prof. Dr. Xia Chu,

    Corresponding author
    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
    • State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • Dr. Tingting Chen,

    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • Dr. Jia Ge,

    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • Prof. Ruqin Yu

    1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China), Fax: (+86) 731-8882-1916
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  • This work was supported by the NSFC (20975035), “973” National Key Basic Research Program (2007CB310500), and PCSIRT.

Abstract

original image

All systems GO! An intracellular protease sensor is based on the covalent conjugate of graphene oxide and peptide substrates with fluorophore labels. The conjugate can be delivered into live cells and provides specific, high-contrast imaging of caspase-3 activation (see picture; orange=cell penetration peptide, blue/black=caspase-3 peptide probe).

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