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Keywords:

  • click chemistry;
  • fluorescence spectroscopy;
  • nanoparticles;
  • proteins
Thumbnail image of graphical abstract

Tracking single proteins in cells: An optimized substrate based on a click reaction was designed for specific, irreversible targeting of nanoparticles to proteins fused to an engineered haloalkane dehalogenase (HaloTag) in the cytoplasm of life cells. Thus, proteins in the membrane of mitochondria could be tracked over extended times and with nanometer resolution (see picture).