Gold Nanoparticles Presenting Hybridized Self-Assembled Aptamers That Exhibit Enhanced Inhibition of Thrombin

Authors

  • Yen-Chun Shiang,

    1. Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617 (Taiwan), Fax: (+886) 2-3366-1171
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  • Chia-Lun Hsu,

    1. Institute of Bioscience and Biotechnology, Center of Excellence for Marine Bioenvironment and Biotechnology (CMBB), National Taiwan Ocean University 2, Pei-Ning Rd, Keelung 20224 (Taiwan), Fax: (+886) 2-2462-2034
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  • Prof. Chih-Ching Huang,

    Corresponding author
    1. Institute of Bioscience and Biotechnology, Center of Excellence for Marine Bioenvironment and Biotechnology (CMBB), National Taiwan Ocean University 2, Pei-Ning Rd, Keelung 20224 (Taiwan), Fax: (+886) 2-2462-2034
    • Institute of Bioscience and Biotechnology, Center of Excellence for Marine Bioenvironment and Biotechnology (CMBB), National Taiwan Ocean University 2, Pei-Ning Rd, Keelung 20224 (Taiwan), Fax: (+886) 2-2462-2034
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  • Prof. Huan-Tsung Chang

    Corresponding author
    1. Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617 (Taiwan), Fax: (+886) 2-3366-1171
    • Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617 (Taiwan), Fax: (+886) 2-3366-1171
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  • This study was supported by the National Science Council of Taiwan under contract NSC 98-2113M-002-011-MY3.

Abstract

original image

DNA hybridization of thrombin-binding aptamers (TBAs) to form a self-assembled arranged monolayer on gold nanoparticles (Au NPs) was used to control the coagulation activity of thrombin. The hTBA15/hTBA29/cDNA-Au NPs provided flexibility and a suitable orientation and distance between the hTBA15 and hTBA29 units for bivalent binding, allowing strong interactions with thrombin and extremely high anticoagulant potency.

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