Get access

Observation of In Vivo Cytochrome-Based Electron-Transport Dynamics Using Time-Resolved Evanescent Wave Electroabsorption Spectroscopy

Authors

  • Toshihiko Shibanuma,

    1. Department of Applied Chemistry, School of Engineering, The University of Tokyo (Japan)
    Search for more papers by this author
  • Dr. Ryuhei Nakamura,

    1. Department of Applied Chemistry, School of Engineering, The University of Tokyo (Japan)
    Search for more papers by this author
  • Yuichiro Hirakawa,

    1. Department of Applied Chemistry, School of Engineering, The University of Tokyo (Japan)
    Search for more papers by this author
  • Prof. Dr. Kazuhito Hashimoto,

    Corresponding author
    1. HASHIMOTO Light Energy Conversion Project, ERATO/JST, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904 (Japan)
    2. Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    • HASHIMOTO Light Energy Conversion Project, ERATO/JST, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904 (Japan)
    Search for more papers by this author
  • Prof. Dr. Kazuyuki Ishii

    Corresponding author
    1. HASHIMOTO Light Energy Conversion Project, ERATO/JST, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904 (Japan)
    2. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 (Japan)
    • HASHIMOTO Light Energy Conversion Project, ERATO/JST, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904 (Japan)
    Search for more papers by this author

  • This work was financially supported by Exploratory Research for Advanced Technology (ERATO) program of the Japan Science and Technology Agency (JST). We acknowledge Dr. K. Watanabe (UT, ERATO/JST) for discussions about strain PV-4.

Abstract

original image

Morning light: In vivo photodissociation of CO from bacterial c-type cytochromes yields a redox-active Fe2+ form, which can be oxidized at an electrode surface to the Fe3+ form. Reduction by electrons from the metabolic pathway regenerates the Fe2+ form (see picture). Spectroscopic monitoring of this process yields information on the in vivo respiratory electron-transport dynamics.

Get access to the full text of this article

Ancillary