Communication

Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study†

  1. Christen Besanceney-Webler1,‡,
  2. Hao Jiang1,‡,
  3. Tianqing Zheng1,
  4. Lei Feng1,
  5. David Soriano del Amo1,
  6. Wei Wang1,
  7. Liana M. Klivansky3,
  8. Prof. Dr. Florence L. Marlow2,*,
  9. Dr. Yi Liu3,*,
  10. Prof. Dr. Peng Wu1,*

Article first published online: 14 JUL 2011

DOI: 10.1002/anie.201101817

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 35, pages 8051–8056, August 22, 2011

Additional Information

How to Cite

Besanceney-Webler, C., Jiang, H., Zheng, T., Feng, L., Soriano del Amo, D., Wang, W., Klivansky, L. M., Marlow, F. L., Liu, Y. and Wu, P. (2011), Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study. Angew. Chem. Int. Ed., 50: 8051–8056. doi: 10.1002/anie.201101817

Author Information

  1. 1

    Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461 (USA)

  2. 2

    Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)

  3. 3

    The Molecular Foundry, Lawrence Berkeley National Laboratory, One Cyclotron Rd, MS 67R6110, Berkeley, CA 94720 (USA)

  1. ‡

    These authors contributed equally to this work.

*Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)

  1. †

    This work was supported by the National Institutes of Health grants GM080585 and GM093282 (P.W.). C.B. is supported by the NIH training grant T32 GM007491. Part of the ligand synthesis was performed as a User Project at the Molecular Foundry, Lawrence Berkeley National Laboratory, which was supported by the Office of Science, Office of Basic Energy Sciences, U.S. Department of Energy, under contract DE-AC02-05 CH11231.

Publication History

  1. Issue published online: 17 AUG 2011
  2. Article first published online: 14 JUL 2011
  3. Manuscript Received: 14 MAR 2011

Funded by

  • National Institutes of Health. Grant Numbers: GM080585, GM093282, T32 GM007491
  • Office of Science, Office of Basic Energy Sciences, U.S. Department of Energy. Grant Number: DE-AC02-05 CH11231

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Keywords:

  • alkynes;
  • azides;
  • bioconjugation;
  • bioorthogonal reactions;
  • click chemistry
Thumbnail image of graphical abstract

Raising the bar: The efficacy of bioorthogonal reactions for bioconjugation has been thoroughly evaluated in four different biological settings. Powered by the development of new biocompatible ligands, the copper-catalyzed azide–alkyne cycloaddition (see picture) has brought about unsurpassed bioconjugation efficiency, and thus it holds great promise as a highly potent and adaptive tool for a broader spectrum of biological applications.

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