Get access

Transmembrane Domain Peptide/Peptide Nucleic Acid Hybrid as a Model of a SNARE Protein in Vesicle Fusion

Authors

  • Antonina S. Lygina,

    1. Institut für Organische und Biomolekulare Cehmie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen (Germany)
    Search for more papers by this author
  • Karsten Meyenberg,

    1. Institut für Organische und Biomolekulare Cehmie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen (Germany)
    Search for more papers by this author
  • Prof. Dr. Reinhard Jahn,

    1. Abteilung Neurobiologie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen (Germany)
    Search for more papers by this author
  • Prof. Dr. Ulf Diederichsen

    Corresponding author
    1. Institut für Organische und Biomolekulare Cehmie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen (Germany)
    • Institut für Organische und Biomolekulare Cehmie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen (Germany)
    Search for more papers by this author

  • We thank Heinrich Prinzhorn for DLS measurements. Generous support from the Deutsche Forschungsgemeinschaft DFG (SFB 803) is gratefully acknowledged.

Abstract

original image

Artificial models of the natural SNARE proteins containing the native linker regions (light blue/gray) and the transmembrane domains (TMDs) of synatobrevin (Syb, violet) and syntaxis-1A (Sx, orange) along with a peptide nucleic acid (PNA) recognition motif can align with either an antiparallel (see picture) or parallel orientation of the interacting strands. These hybrids induce the hemifusion and partial bilayer fusion of phospholipid vesicles.

Get access to the full text of this article

Ancillary