Single Gold Nanoparticles as Real-Time Optical Probes for the Detection of NADH-Dependent Intracellular Metabolic Enzymatic Pathways

Authors

  • Lei Zhang,

    1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
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    • These authors contributed equally to this work.

  • Dr. Yang Li,

    1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
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    • These authors contributed equally to this work.

  • Dr. Da-Wei Li,

    1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
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  • Chao Jing,

    1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
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  • Prof. Xiaoyuan Chen,

    1. Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892 (USA)
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  • Min Lv,

    1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800 (P. R. China)
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  • Prof. Qing Huang,

    1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800 (P. R. China)
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  • Prof. Yi-Tao Long,

    Corresponding author
    1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
    • Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21-6425-0032
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  • Prof. Itamar Willner

    1. Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904 (Israel)
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  • This research is supported by the Major Research Plan of National Natural Science Foundation of China (91027035), Key Program of National Natural Science of China (20933007), and Shanghai Pujiang Program Grant of China (09PJ1403300).

Abstract

original image

A coat of many colors: Plasmon resonance Rayleigh scattering (PRRS) spectroscopy is applied to the in-situ monitoring of the NADH-dependent growth of Au@Cu core–shell nanoparticles (NPs). The scattering signal of the AuNPs is red shifted when they are coated with Cu (see scheme). In conjunction with dark-field microscopy, a colorimetric NADH-dependent biosensor was constructed and tested to screen an anticancer drug affecting cell NADH metabolism.

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