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Keywords:

  • biocatalysis;
  • directed evolution;
  • enantioselectivity;
  • flow cytometry;
  • in vivo selection
Thumbnail image of graphical abstract

The “carrot and stick” principle could be combined with cell sorting to enable the selection of enantioselective esterase variants from a mutant library. Hence, the enormous diversity generated in directed evolution experiments is now easily accessible by this high-throughput system. In line with the principle, the hydrolysis of 1 glycerin supports cell growth, whereas the hydrolysis of 2 leads to cell death.