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Keywords:

  • bioconjugation;
  • EPR spectroscopy;
  • Mannich reaction;
  • proteins;
  • radicals
Thumbnail image of graphical abstract

Keeping tabs on tyrosine: A three-component Mannich-type reaction extends the scope of site-directed spin labeling by selectively labeling the unique tyrosine residue of CP12 protein (see picture), as was confirmed by mass spectrometry. EPR spectroscopy of the labeled protein showed a very high mobility of the probe, which remained very mobile after complex formation with GAPDH.