Communication

Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins†

  1. Magali Lorenzi1,2,
  2. Carine Puppo1,2,
  3. Dr. Régine Lebrun3,
  4. Sabrina Lignon3,
  5. Dr. Valérie Roubaud4,
  6. Dr. Marlène Martinho1,2,
  7. Dr. Elisabetta Mileo4,
  8. Prof. Paul Tordo4,
  9. Prof. Sylvain R. A. Marque4,*,
  10. Dr. Brigitte Gontero1,2,
  11. Prof. Bruno Guigliarelli1,2,
  12. Dr. Valérie Belle1,2,*

Article first published online: 24 AUG 2011

DOI: 10.1002/anie.201102539

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 39, pages 9108–9111, September 19, 2011

Additional Information

How to Cite

Lorenzi, M., Puppo, C., Lebrun, R., Lignon, S., Roubaud, V., Martinho, M., Mileo, E., Tordo, P., Marque, S. R. A., Gontero, B., Guigliarelli, B. and Belle, V. (2011), Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins. Angew. Chem. Int. Ed., 50: 9108–9111. doi: 10.1002/anie.201102539

Author Information

  1. 1

    Bioénergétique et Ingénierie des Protéines UPR 9036, CNRS

  2. 2

    Aix-Marseille Université, Institut de Microbiologie de la Méditérranée, 31 chemin J. Aiguier, 13402 Marseille Cedex 20 (France)

  3. 3

    Plateforme Protéomique, IFR88, IBiSA Marseille-Protéomique, CNRS, Institut de Microbiologie de la Méditérranée, 13402 Marseille Cedex 20 (France)

  4. 4

    Laboratoire Chimie Provence, LCP-UMR 6264, Université de Provence case 521, Avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20 (France)

*Laboratoire Chimie Provence, LCP-UMR 6264, Université de Provence case 521, Avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20 (France)

  1. †

    This work was supported by the Agence Nationale de la Recherche ANR SPINFOLD no. 09-BLAN-0100, the Centre National de la Recherche Scientifique (CNRS) and Aix-Marseille University. M.L. is grateful to the French Minister of Research for her PhD fellowship. We thank M. Joint and M. Belghazi for help in the MS and MS/MS data collection and analyses on the Ultraflex MALDI-ToFToF, IFR Jean-Roche, MaP. We thank C. Chendo and V. Monnier from the Spectropole of Aix-Marseille University for the ESI-MS study. We thank E. Etienne for the simulation of the EPR spectra and A. Cornish Bowden for revising the English manuscript.

Publication History

  1. Issue published online: 15 SEP 2011
  2. Article first published online: 24 AUG 2011
  3. Manuscript Revised: 3 JUN 2011
  4. Manuscript Received: 12 APR 2011

Funded by

  • Agence Nationale de la Recherche ANR SPINFOLD. Grant Number: 09-BLAN-0100
  • Centre National de la Recherche Scientifique
  • CNRS

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