Communication

Aplysqualenol A Binds to the Light Chain of Dynein Type 1 (DYNLL1)†

  1. Brunilda Vera1,
  2. Prof. Abimael D. Rodríguez1,*,
  3. Dr. James J. La Clair2,*

Article first published online: 8 JUL 2011

DOI: 10.1002/anie.201102546

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 35, pages 8134–8138, August 22, 2011

Additional Information

How to Cite

Vera, B., Rodríguez, A. D. and La Clair, J. J. (2011), Aplysqualenol A Binds to the Light Chain of Dynein Type 1 (DYNLL1). Angew. Chem. Int. Ed., 50: 8134–8138. doi: 10.1002/anie.201102546

Author Information

  1. 1

    Department of Chemistry, University of Puerto Rico, P.O. Box 23346, UPR Station, San Juan, 00931–3346 (Puerto Rico)

  2. 2

    Xenobe Research Institute, P.O. Box 3052, San Diego, CA 92163-1052 (USA) http://www.xenobe.org

*Department of Chemistry, University of Puerto Rico, P.O. Box 23346, UPR Station, San Juan, 00931–3346 (Puerto Rico)

  1. †

    We gratefully acknowledge the financial support of the National Institutes of Health (1SC1GM086271 and 1SC1GM086271-01A1). We wish to thank Ignacio Rodríguez-Crespo (Universidad Complutense de Madrid) for providing a plasmid containing the DYNLL1 gene, Dr. J. Beld and M. D. Burkart (UC San Diego) for assistance in the production of the recombinant DYNLL1, Lauge Farnes and Chambers Hughes (UC San Diego) for assistance with the capillary NMR analyses, and Jan Vincente (UPR) for assisting with underwater photography.

Publication History

  1. Issue published online: 17 AUG 2011
  2. Article first published online: 8 JUL 2011
  3. Manuscript Received: 12 APR 2011

Funded by

  • National Institutes of Health. Grant Numbers: 1SC1GM086271, 1SC1GM086271-01A1

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Keywords:

  • aplysqualenol A;
  • bidirectional affinity chromatography;
  • dyneins;
  • cell screening;
  • natural products
Thumbnail image of graphical abstract

A bidirectional affinity system has been developed for the identification of cancer-related natural products and their biological targets. Aplysqualenol A is thus selectively identified as a ligand of the dynein light chain. The use of forward and reverse affinity methods suggests that both small-molecule isolation and target identification can be conducted using conventional molecular biological methods.

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