• Open Access

Label-Free Detection of Single-Base Mismatches in DNA by Surface-Enhanced Raman Spectroscopy

Authors

  • Evanthia Papadopoulou,

    1. Innovative Molecular Materials Group, School of Chemistry & Chemical Engineering, Queen's University, Belfast BT9 5AG (UK)
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  • Dr. Steven E. J. Bell

    Corresponding author
    1. Innovative Molecular Materials Group, School of Chemistry & Chemical Engineering, Queen's University, Belfast BT9 5AG (UK)
    • Innovative Molecular Materials Group, School of Chemistry & Chemical Engineering, Queen's University, Belfast BT9 5AG (UK)
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  • E. P. thanks Queen's University, Belfast for the award of a McClay Trust Studentship.

Abstract

original image

Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single-base mismatches in short (25-mer and 23-mer) strands. Subtracting the spectra of different DNA sequences results in difference spectra that contain features corresponding to the exchanged nucleotides.

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