SEARCH

SEARCH BY CITATION

Keywords:

  • fluorescent probes;
  • nonnatural amino acids;
  • phosphorylation;
  • STAT proteins
Thumbnail image of graphical abstract

Roving reporter: A reversible biosensor reports the phosphorylation status of full-length substrate protein STAT3 by genetically incorporating 7-hydroxycoumarin into its phosphotyrosine binding pocket (see picture). A large fluorescence increase with characteristic emission and excitation properties results as a response to phosphorylation of STAT3 in vitro and endogenously in nuclear extracts.