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Nanostructured Immunosensor for Attomolar Detection of Cancer Biomarker Interleukin-8 Using Massively Labeled Superparamagnetic Particles


  • This research was financially supported by the NCRR/NIH (grant P20RR016457) awarded to B.S.M. and in part by the NIEHS/NIH (US PHS grant ES013557) awarded to J.F.R. Manuscript preparation was supported in part by the Intramural Research Program of NIDCR/NIH.


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A sensitive issue: Superparamagnetic particle–antibody conjugates enable detection of interleukin-8 (IL-8) in serum at a level of 1 fg mL−1 (ca. 100 aM). Ultrahigh sensitivity is facilitated by a nanostructured sensor platform coupled with secondary antibody–magnetic bead–horseradish peroxidase conjugate (Ab2–MB–HRP) with about 500 000 HRP labels (see picture).