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Impact of Strand Length on the Stability of Parallel-β-Sheet Secondary Structure

Authors

  • Dr. Felix Freire,

    1. Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
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    • These authors contributed equally to this work.

  • Aaron M. Almeida,

    1. Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
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    • These authors contributed equally to this work.

  • Dr. John D. Fisk,

    1. Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
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  • Jay D. Steinkruger,

    1. Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
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  • Prof. Samuel H. Gellman

    Corresponding author
    1. Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
    • Department of Chemistry, University of Wisconsin–Madison, 1101 University Av., Madison, WI 53726 (USA)
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  • This research was supported by the NIH (GM-061238). F.F. was supported in part by an MEC-Fulbright Postdoctoral Fellowship. J.D.F. was supported in part by a biophysics training grant from NIGMS. NMR spectrometers were purchased with partial support from NIH and NSF.

Abstract

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Molecular design can be used to investigate whether the parallel-β-sheet secondary structure is subject to length-dependent cooperative stabilization along the strand direction (see picture). Extending strands by adding high-propensity residues (B) leads to a steady increase in parallel-β-sheet stability, but inclusion of low-propensity residues (A) can negate this stabilization.

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