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We are indebted to Dr. Michael G. Hoesl and Dr. Lars Merkel for critical reading of this manuscript and numerous productive discussions on this subject.
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On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall†
Article first published online: 24 JUN 2011
DOI: 10.1002/anie.201103010
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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How to Cite
Acevedo-Rocha, C. G. and Budisa, N. (2011), On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall. Angew. Chem. Int. Ed., 50: 6960–6962. doi: 10.1002/anie.201103010
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Publication History
- Issue published online: 21 JUL 2011
- Article first published online: 24 JUN 2011
- Manuscript Received: 2 MAY 2011
- Abstract
- Article
- References
- Cited By
Keywords:
- chemical evolution;
- nucleobases;
- synthetic biology;
- xenobiology
An automated system for the continuous cultivation of cells in suspension has been used to globally change the composition of the DNA genome of E. coli. Thymine was replaced almost completely by 5-chlorouracil through artificial evolution of cells, during which the bacterial genome accumulated hundreds of mutations under permanent conditions of proliferation and selection. The resulting 5-chlorouracil-adapted descendants had the ability to grow on 5-chlorouracil.