These authors contributed equally to this work.
Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification†
Article first published online: 24 AUG 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 50, Issue 40, pages 9378–9381, September 26, 2011
How to Cite
Budin, G., Yang, K. S., Reiner, T. and Weissleder, R. (2011), Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification. Angew. Chem. Int. Ed., 50: 9378–9381. doi: 10.1002/anie.201103273
This work was supported by the National Institutes of Health (NIH) grant number RO1EB010011, K.S. was supported by a NIH grant T32-CA79443 and T.R. was supported by a grant from the German Academy of Sciences Leopoldina (LPDS 2009-24). We thank Joshua Dunham and Alex Zaltsman for image processing, and Dr. Robert Yang and Prof. Peter Sorger for assistance with cellWoRx and Image Rail.
- Issue published online: 21 SEP 2011
- Article first published online: 24 AUG 2011
- Manuscript Revised: 13 JUL 2011
- Manuscript Received: 12 MAY 2011
- National Institutes of Health (NIH). Grant Numbers: RO1EB010011, T32-CA79443
- German Academy of Sciences Leopoldina. Grant Number: LPDS 2009-24
- cell cycle;
- live cell imaging;
- polo-like kinase
Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins.