Control of Circadian Phase by an Artificial Zinc Finger Transcription Regulator


  • We thank Dr. H. Tei for kindly providing reporter plasmids and Dr. J.-M. Fustin for the constructive discussion. This work was partially supported by PRESTO, JST (Japan); Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MECSST) (Japan); Hayashi Memorial Foundation for Female Natural Scientists to M.I.; and The Special Promotion Grant from MECSST to H.O. A.N. is grateful for the JSPS Research Fellowship for Young Scientists.


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I got rhythm: The circadian clock is driven by transcription–translation feedback loops. The circadian time can be altered by creating an artificial zinc finger protein specifically binding to the glucocorticoid responsive element (GRE) on the Period1 promoter (see picture; DD=destabilizing domain, AD=activation domain). This artificial protein directly controls the clock machinery.