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Photolyase-like Repair of Psoralen-Crosslinked Nucleic Acids


  • This work was generously supported by the Deutsche Akademie der Naturforscher Leopoldina (Bundesministerium für Bildung und Forschung BMBF-LPD 9901/8-158) and the ETH Zürich.


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Psoralen-derived photolesions are efficiently repaired by a photolyase-like mechanism. The removal of the interstrand crosslink by photoelectron injection confers control over biochemical processes by light (see picture). This paves the way to new, site-selective uncaging applications, as demonstrated with a primer extension assay.