Communication

Vibralactone as a Tool to Study the Activity and Structure of the ClpP1P2 Complex from Listeria monocytogenes†

  1. Evelyn Zeiler1,
  2. Dr. Nathalie Braun2,
  3. Dr. Thomas Böttcher3,4,
  4. Andreas Kastenmüller2,
  5. Prof. Dr. Sevil Weinkauf2,
  6. Prof. Dr. Stephan A. Sieber1,*

Article first published online: 22 SEP 2011

DOI: 10.1002/anie.201104391

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 50, Issue 46, pages 11001–11004, November 11, 2011

Additional Information

How to Cite

Zeiler, E., Braun, N., Böttcher, T., Kastenmüller, A., Weinkauf, S. and Sieber, S. A. (2011), Vibralactone as a Tool to Study the Activity and Structure of the ClpP1P2 Complex from Listeria monocytogenes. Angew. Chem. Int. Ed., 50: 11001–11004. doi: 10.1002/anie.201104391

Author Information

  1. 1

    Department Chemie, Center for Integrated Protein Science CIPSM, Institute of Advanced Studies IAS, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

  2. 2

    Center for Integrated Protein Science Munich CIPSM, Department of Chemistry, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

  3. 3

    AVIRU, EXIST Transfer of Research, OC II, Lichtenbergstrasse 4, 85747 Garching (Germany)

  4. 4

    Current address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA (USA)

*Department Chemie, Center for Integrated Protein Science CIPSM, Institute of Advanced Studies IAS, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

  1. †

    We thank Mona Wolff for excellent scientific support and Wolfgang Steglich for helpful discussions. E.Z. was supported by the SFB749 and by the TUM-GS. E.Z. thanks Quan Zhou for helpful discussions. T.B. was supported by the German National Academic Foundation and by an EXIST technology transfer grant of the Federal Ministry of Economics and Technology (BMWi); S.A.S. was supported by the Deutsche Forschungsgemeinschaft (Emmy Noether), SFB749, FOR1406, an ERC starting grant, and the Center for Integrated Protein Science Munich CIPSM. S.W. and N.B. were funded by the DFG (SFB594) and CIPSM.

Publication History

  1. Issue published online: 9 NOV 2011
  2. Article first published online: 22 SEP 2011
  3. Manuscript Received: 24 JUN 2011

Funded by

  • Federal Ministry of Economics and Technology (BMWi)
  • Deutsche Forschungsgemeinschaft
  • ERC starting grant
  • Center for Integrated Protein Science Munich CIPSM

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Keywords:

  • electron microscopy;
  • natural products;
  • protein structures;
  • proteomics;
  • vibralactone
Thumbnail image of graphical abstract

The Clp proteolytic machinery has important functions in many bacteria such as L. monocytogenes. Some organisms encode for two uncharacterized ClpP isoforms. Vibralactone was used to study the activity and assembly of ClpP1 and ClpP2 subunits in a hetero-oligomeric complex. Electron microscopic images reveal that the tetradecameric assembly is made up of two stacked homoheptameric ClpP1 and ClpP2 rings.

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