Simultaneous Photographing of Oxygen and pH In Vivo Using Sensor Films


  • The authors are grateful to the Deutsche Forschungsgemeinschaft for grants WO 669/9-1 and BA 3410/4-1, and the Novartis Foundation Novartis Graduate Scholarship (to S.S.). X.W. thanks the German Academic Exchange Service (DAAD) for a grant. The authors thank Josef Schroeder and Heiko Siegmund (Institute of Pathology) for electron microscopic images, Tim Maisch, Johannes Regensburger, and Francesco Santarelli (Department of Dermatology) for keratinocyte cultures and singlet oxygen detection, Karla Lehle and Christina Leykauf (Department of Cardiothoracic Surgery) for cell cultures and cytotoxicity tests, Judith Stolwijk (Institute of Analytical Chemistry, Chemo- and Biosensors) for confocal microscopic imaging, and Timo Lauber and Dietmar Schreml for their help with video processing.


original image

Two kinds of imaging: A fluorescent sensor membrane is presented that responds to pH and to pO2 at different emission wavelengths. The fluorescence intensities can be imaged with a digital camera using the red-green-blue (RGB) data storage option.