High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery


  • The research described in this article was funded by the Energy Biosciences Institute.


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Combined efforts: A high-throughput protein-expression and screening method (HIGH method, see picture) provides a rapid approach to the discovery of active glycoside hydrolases in environmental samples. HIGH screening combines cloning, protein expression, and enzyme hydrolysis in one pot; thus, the entire process from gene expression to activity detection requires only three hours.