Insights into Neuronal Cell Metabolism Using NMR Spectroscopy: Uridyl Diphosphate N-Acetyl-Glucosamine as a Unique Metabolic Marker

Authors


  • The authors thank the DFG (KFO 126, B1) and the University of Lübeck for financial support. The DFG and the state of Schleswig-Holstein are thanked for a grant for the cryogenic probe (HBFG 101/192-1). We thank Dr. Olaf Jöhren and Dr. Hannelore Peters for stimulating discussions.

Abstract

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Making the switch: Compounds 1 and 2 are used as metabolic markers for NMR detection. When neuronal cells switch to a glycolytic state, an uneven distribution of 13C in the N-acetyl group results, thus giving a mixture of the metabolites 1 and 2. It is therefore possible to monitor flux through different metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, and the hexosamine biosynthetic pathway, using a single molecule.

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