Topological Characterization of Nucleic Acid G-Quadruplexes by UV Absorption and Circular Dichroism

Authors

  • Dr. Andreas Ioannis Karsisiotis,

    1. School of Biomedical Sciences, University of Ulster, Coleraine, Cromore Road, BT52 1SA (UK)
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  • Dr. Nason Ma'ani Hessari,

    1. School of Biomedical Sciences, University of Ulster, Coleraine, Cromore Road, BT52 1SA (UK)
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  • Prof. Ettore Novellino,

    1. Dipartimento di Chimica Farmaceutica e Tossicologica, Università degli Studi di Napoli “Federico II”, Napoli (Italy)
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  • Prof. Gian Piero Spada,

    Corresponding author
    1. Dipartimento di Chimica Organica “A. Mangini”, Alma Mater Studiorum—Università di Bologna via San Giacomo 11, 40126 Bologna (Italy)
    • Dipartimento di Chimica Organica “A. Mangini”, Alma Mater Studiorum—Università di Bologna via San Giacomo 11, 40126 Bologna (Italy)
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  • Prof. Antonio Randazzo,

    Corresponding author
    1. Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli “Federico II” via D. Montesano 49, 80131 Napoli (Italy)
    • Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli “Federico II” via D. Montesano 49, 80131 Napoli (Italy)
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  • Dr. Mateus Webba da Silva

    Corresponding author
    1. School of Biomedical Sciences, University of Ulster, Coleraine, Cromore Road, BT52 1SA (UK)
    • School of Biomedical Sciences, University of Ulster, Coleraine, Cromore Road, BT52 1SA (UK)
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  • This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC Grant number BB/H005692/1). We gratefully acknowledge the European Commission through COST Action MP0802 for offering a stimulating environment for discussions on the topic.

Abstract

original image

Four-fold: Not just the identification, but also the rapid characterization and classification of nucleic acid G-quadruplexes into topology groups is feasible utilizing UV and circular dichroism (CD) spectroscopy (see picture). It is now possible to utilize inexpensive UV spectroscopy to achieve the same level of characterization previously only possible with CD spectroscopy.

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